TYROSINASE INHERITANCE IN STREPTOMYCES SCABIES II
نویسندگان
چکیده
منابع مشابه
Tyrosinase Inheritance in Streptomyces Scabies. Ii. Induction of Tyrosinase Deficiency by Acridine Dyes.
Gregory, Kenneth F. (Ontario Agricultural College, Guelph, Ontario, Canada), and Jay C. C. Huang. Tyrosinase inheritance in Streptomyces scabies. II. Induction of tyrosinase deficiency by acridine dyes. J. Bacteriol. 87:1287-1294. 1964.-Growth in minimal medium containing 1 mug of acriflavine per ml resulted in a large increase (up to 62%) in the frequency of tyrosinase-deficient (tye(-)) mutan...
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Site-directed mutagenesis was used to determine the functional role of several residues of Streptomyces glaucescens tyrosinase. Replacement of His-37, -53, -193 or -215 by glutamine yields albino phenotypes, as determined by expression on melanin-indicator plates. The purified mutant proteins display no detectable oxy-enzyme and increased Cu lability at the binuclear active site. The carbonyl d...
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Tyrosinases (monophenol, o-diphenol:oxygen oxidoreductase, EC (1.14.18.1) are copper-containg enzymes which catalyze the o-hydroxylation of monophenols and subsequent oxidation of o-diphenols to quinones. The enzymes are involved in the pigmentation and are important factors in wound healing and primary immune response. Tyrosinases are found in prokaryotic and eukaryotic microorganisms, in mamm...
متن کاملCloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
In two separate studies a BclI-generated DNA fragment coding for the enzyme tyrosinase, responsible for melanin synthesis, was cloned from Streptomyces antibioticus DNA into two SLP1.2-based plasmid vectors (pIJ37 and pIJ41) to generate the hybrid plasmids, designated pIJ700 and pIJ701, using S. lividans 66 as the host. The fragment (1.55 kb) was subcloned into the multicopy plasmid pIJ350 (whi...
متن کاملبرهمکنش نماتد ریشه‌گرهی Meloidogyne javanica)) و باکتری عامل جرب معمولی (Streptomyces scabies) روی سیب زمینی
باکتر‌ی‌های عامل جرب معمولی (Streptomyces sp.) و نماتد‌های ریشه‌گرهی(Meloidogyne sp.)از جمله عوامل بیماریزای مهم سیبزمینی هستند که هر یک به تنهایی خسارت قابل توجهی به این محصول وارد میسازند.به منظور بررسی برهمکنش اینبیمارگرها، مایه‌زنی سیبزمینی (رقم اگریا) با باکتری S. scabies و نماتد M. javanica به تنهایی و در ترکیب با هم در زمان‌های مختلف بصورت طرح کاملاً تصادفی با هفت تیمار و شش تکرار در گ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.87.6.1287-1294.1964